Western Blot Detection System

Quick visualization precise quantitation single probe detection or multiplexing we offer a range of reagents kits and instruments for western blot detection and analysis.

Western blot detection system.

The nano glo hibit blotting system visualizes hibit tagged proteins on blots at subpicogram levels. Bio rad s v3 western workflow facilitates speed and validation at each step of a western blotting experiment from running gels to quantifying proteins. Western blotting is a tried and true technique in the life science lab for separating proteins based on their molecular weight. Find step by step protocols and helpful tips on equipment membranes transfer conditions and detection methods.

The method is based on the construction of an antibody protein complex with specific antibodies that bind to proteins immobilized on a membrane. Learn more about western blotting techniques. The western blot is extensively used in biochemistry for the qualitative detection of single proteins and protein modifications such as post translational modifications at least 8 9 of all protein related publications are estimated to apply western blots. Western blot is a well established and widely used technique for the detection and analysis of proteins.

The immunodetection phase of western blotting consisting of blocking antibody incubations and washing steps may be subject to experimental variability. Pierce ecl western blotting substrate provides reliability and performance equivalent to other standard ecl substrates for detection of hrp enzyme activity. It is used as a general method to identify the presence of a specific single protein within a complex mixture of proteins. We offer comprehensive protein detection solutions designed for your western blotting success.

It enables the detection of picogram amounts of antigen and allows for easy detection of hrp using photographic or other imaging methods. Protein separation and analysis.